Detection of ACE2 Protein Levels Helps Track SARS-CoV-2 Infection

The research team used the data from BIAL Biobank, integrated with the Spanish National Biobank Network and the Valencian Biobank Network. Fifty-nine patients (24 were women and 35 men) with a mean age of 64 years who had got a positive RT-PCR test result were examined in the study.

Forty-eight people from this group had a moderate infection, while the remaining eleven people experienced respiratory failure and sought invasive mechanical ventilation and/or intensive care unit treatment. Two additional groups, one with 17 participants (aged 34 to 85 years), included patients with influenza A virus pneumonia, and the other consisted of 26 disease-free controls (14 women and 12 men) aged 34-85 years.

Immunoprecipitation and western blotting analyses were carried out to identify the ACE2 species in human plasma received from all the participants. The changes in ACE2 species were also examined in serum samples from mice that are genetically manipulated to have a human version of ACE2 protein.

All these analyses proved that both normal and truncated forms of ACE2 present in plasma could be used as a good biomarker of the evolution of coronavirus infection.

“In this work, we have studied the plasma levels of the coronavirus receptor, the ACE2 protein, and we have been able to determine that there are different forms of the protein in plasma, and that part of the soluble ACE2 are proteolytic fragments of the ACE2 receptor, generated subsequently to interaction with the virus. The full-length protein is also found in plasma, which provides information about tissue affection during infection,” explained Javier Sez-Valero, lead investigator of the study.

Source: Medindia